Fig. 6. FTY720-P and PKC activates ERK. Effect of (A) FTY720-P (7.5nM. 2hours) and (B) PMA (100nM, 2hours) on Na⁺/K⁺ ATPase activity in cells pre-treated for 30 min with the ERK inhibitor PD98059 (50 µM). (C) Effect of PKC and ERK inhibition on the Protein expression of p-ERK. Values are normalized to GAPDH using Image lab software. The blot is representative of an experiment repeated 3 times. Values are means ± SEM of at least 3 observations. Bars not sharing a common letter are considered significantly different from each other at p<0.01.